Supported Liquid Extraction Microplate for Biological Fluids
26 Jul 2017
Porvair Sciences has launched a 96-well Supported Liquid Extraction (SLE) microplate designed for high recovery extraction of analytes from biological fluids.
Using the Porvair SLE plate, high analyte recovery - free of proteins and phospholipids - is routinely obtainable without the need for offline steps such as protein precipitation.
Supported Liquid Extraction is a separation technique similar to traditional liquid-liquid extraction (LLE) which uses water and an immiscible solvent to extract analytes. In SLE, the aqueous sample is immobilised on an inert support such as diatomaceous earth, and the organic phase flows through the support, eliminating problems such as emulsion formation.
Based upon the automation compatible Microlute 96-well format, the Porvair SLE plate provides fast, reproducible and economical sample clean-up when used with a simple vacuum manifold or positive pressure device. Proprietary technology in the plate ensures that samples will not drip or breakthrough until pressure or vacuum are applied, allowing longer dwell time in the plate wells.
The large pore size and high pore volume of the diatomaceous earth inert support, combined with a wide pH working range (1-13), allows the separation of viscous aqueous solutions such as blood, plasma and serum which may contain phospholipids and proteins. Demonstrating high recovery rates for small polar and non-polar compounds, the plate provides a suitable sample clean-up device for physiological fluids prior to analysis by LC/MS.