Porvair Sciences’ Chromatrap Lysis and Hypotonic buffers can enable researchers to explore and understand the underlying processes and mechanisms that drive biological organisms.
The buffers have been formulated to act rapidly, gently and efficiently, ensuring reproducible results with high quality chromatin yields from various sample types. With chromatin being at the heart of Chromatin Immunoprecipitation (ChIP), the extraction and purification of chromatin is a vital starting point for epigenetics research.
The Hypotonic Buffer has been widely proven to provide effective disruption of cell membranes through swelling of the cells. Benefiting from its optimised proprietary composition, it delivers effective lysis of cell membranes to aid in the release of cell nuclei from a wide range of different organisms.
Chromatrap Lysis Buffers are formulated to ensure the nuclear membrane is disrupted releasing a high yield of nuclear material for research. The buffer for enzymatic shearing provides users with a complete lysis buffer for the disruption of nuclear cell membranes containing a milder detergent than that required for mechanical fragmentation.
Alternatively, the Lysis buffer for sonication is formulated to ensure effective nuclear lysis and mechanical shearing for a range of different species of organism and is suitable for difficult to lyse cell types and tissues. This detergent-based buffer can be used for the fragmentation of samples by sonication.