Chromatrap has entered into a partnership with Covaris to offer a highly scalable and simple ChIP workflow enabling researchers to easily isolate protein-DNA complexes for downstream applications, such as ChIP-Seq, ChIP-qPCR, Next-generation sequencing (NGS), and Mass Spectrometry (MS).
Pairing the bead-free Chromatrap immunoprecipitation system with the Covaris truChIP and AFA sample preparation technologies enables investigators to rapidly profile protein-DNA interactions of interest. By combining the two technologies, researchers gain access to a compatible and optimised workflow that increases productivity and allows the user to gain better results with higher quality samples.
Chromatrap is designed to eliminate many of the challenges encountered when working with magnetic beads. The rapid centrifugation-based protocol requires only three simple washes using individual spin columns or 96-well plates and can be completed in a single day. In contrast, typical bead-based workflows take around ten hours with more than ten wash steps and incubations.
Using the Covaris Adaptive Focused Acoustics (AFA) technology, researchers can isolate and reproducibly shear soluble chromatin without disrupting the protein-DNA interactions for immunoprecipitation with bead-free Chromatrap Protein A and G ChIP kits.