The Mercodia Glucagon ELISA, which enables users to measure physiologically suppressed levels of Glucagon, is available from Diagenics Ltd.
Requiring only 25μl for human samples and 10μl for animal samples, Mercodia assays contain a unique blocking solution to prevent or minimise matrix interferences. Both the Mercodia Glucagon assays can be used with samples from cultured cells, providing accurate glucagon measurement options for those conducting in vitro research (e.g., conversion of alpha to beta cells or vice versa, human embryonic stem cells to alpha or beta cells, production of islet-like clusters, etc.)
With so many factors potentially influencing the results from an immunoassay, it is extremely important to control every step of the manufacturing process, which is why Mercodia only uses monoclonal antibodies. For the Mercodia Glucagon assays, two highly specific mouse monoclonal antibodies were developed and characterised extensively using ELISA and Biacore techniques.
The 25μl Mercodia Glucagon ELISA is validated for human samples and the 10μl Mercodia Glucagon ELISA has been validated for mouse, rat, and non-human primate samples. The antibodies are controlled and manufactured with the same characteristics in each lot, resulting in assays with very low lot-to-lot variation, year after year.