YMC Europe has described a method that allows rapid analysis of isoflavonoids in food.
As soy is an important source of vegetable oil it contributes essentially to a balanced diet.
Secondary components such as isoflavonoids have a positive effect on the hormonal balance.
However, adverse effects can occur.
The following YMC method for a fast and robust separation of isoflavonoids will facilitate the analysis of these food ingredients.
The analysis time of 30min could be reduced by conventional means of reducing particle size and column dimension (3um, 50 x 4.6mm ID).
To get the same results in terms of the chromatographic behaviour it is important to keep a constant gradient volume.
Increasing the flow rate to 1.5ml/min was necessary to maintain the resolution and elution profile.
Adjusting the gradient profile led to a baseline resolution of the critical peak pair 10 and 11.
This conventional method was then transferred to ultra-fast analysis on a Jasco high-pressure system using 2um particles.
After modifying the chromatographic parameters the flow rate was again increased, which reduced the analysis time in total by a factor of 10.
