Olympus has released the Multichannel 5D Solution module for its Cellsens Dimension software to enable complex multidimensional microscopy experiments.
The module provides advanced, automated acquisition of Z-stacks, multiple fluorescence channels and time-lapse series.
Able to seamlessly integrate with other Olympus hardware and software platforms, the Multichannel 5D Solution module enables the automated acquisition of images in five dimensions (5D) - X, Y, Z, multichannel with transmission overlay and time-lapse.
As a result, the tool can provide researchers with the ability to visualise and analyse multiple features within 3D space, as well as monitoring changes over time, enhancing the ability of the Cellsens Dimension system to carry out customisable and complicated experiments.
When used with the automated Extended Focal Imaging (EFI) tool, it is possible to obtain perfectly focused images by extracting the sharpest features from each Z-stack before reassembling them into a single, clear image.
The EFI tool can be used on-the-fly to display images in real-time.
As well as providing greater physical and temporal flexibility, the module is suited to performing co-localisation studies by facilitating the spectral unmixing of overlapping fluorescence emission signals.
The user interface provides researchers with the ideal workflow, since only the required tools and features are displayed, further simplifying analysis.
In combination with the Olympus Cellsens Dimension Multi-position module and Cellsens Dimension's built in Multiple Image Alignment (MIA) tool, images from adjacent fields can be flawlessly stitched together to create large panoramas that take advantage of all the features provided by the 5D system.
Furthermore, the process can be automated to achieve a level of complex analysis not possible with manual control.
In this way, the 5D platform can be elevated into the 6th Dimension, providing users with more flexibility and functionality.
Designed to offer control and processing for microscope-based experimental systems, the Cellsens Dimension software package provides a range of advanced features, as well as specialised, optional solution modules.
Users can conduct a range of complex and highly sophisticated experiments, from extended focal imaging to multiple image alignment and multi-position imaging.
In addition, live images can be transferred directly to the web using the Netcam Solution, enabling rapid discussion with colleagues, wherever they are in the world.
With the ability to control a range of Olympus and non-Olympus hardware, advanced and precise time-lapse experiments can be conducted, for example.
The resulting time stacks can even be put through kinetic and threshold-based object analysis.
Once data has been obtained, the report composer uses Microsoft Word templates to generate user-defined reports that retrieve images and data directly from the Cellsens database.