Anaspec has announced that it is able to provide Calcein AM at two purity grades - >90 per cent or >95 per cent - as determined by high-performance liquid chromatography (HPLC).
The company also offers the Sensolyte Calcein cell viability assay kit, a rapid and sensitive assay designed to detect viable cells.
The kit provides ample reagents (dye and assay buffer) for 1,000 assays in a 96-well-plate format.
It is used for determining cell viability since the fluorescence intensity of calcein is proportional to the amount of live cells.
Calcein is a photostable reagent and is well retained in the cytoplasm of live cells.
It is not influenced by intracellular pH.
Its bright green fluorescence can be monitored at Ex/Em=494nm/520nm.
Among other reagents, including BCECF, AM and Carboxy-fluorescein diacetate, Calcein AM is the most suitable indicator for staining viable cells due to its low cytotoxicity property.
Calcein AM does not affect cell proliferation or viability.
Calcein AM is used in cell invasion, adhesion, migration and other cell-based assays.