Anaspec has launched a collection of amine- and thiol-reactive 6-isomeric dyes of fluoresceins and rhodamines and as well as Hilyte Fluor dyes for labelling amine- and thiol-modified oligonucleotides.
Fluorescent dye-labelled oligos are widely used as primers for sequencing and PCR reactions and also as probes for fluorescence in-situ hybridisation (FISH).
Double-labelled oligos are used to produce fluorescence resonance energy transfer (FRET) for applications such as real-time PCR assays and automated DNA sequencing.
Synthetic oligos can be modified with amine or thiol groups.
These amine- and thiol-incorporated oligos can then be labelled with amine- and thiol-reactive fluorophores respectively.
N-hydroxysuccinimidyl esters (SE) and isothiocyanates of fluorescein and rhodamine derivatives are used for labelling amine-modified oligos.
Maleimides and iodoacetamides of fluorescein and rhodamine derivatives are used for labelling thiol-modified oligos.
Anaspec's proprietary Hilyte Fluor dyes are available in the SE and maleimide forms.
These dyes have fluorescence emission wavelengths spanning the full visible and near-infrared (NIR) spectrum.
Hilyte Fluor dyes have properties that are claimed to be superior to conventional dyes, with high fluorescence quantum yields, large extinction coefficients, good photostability, low pH sensitivity and improved water solubility.
Anaspec's Hilyte Fluor dyes and classic dyes are good choices for labelling amine- and thiol-modified oligonucleotides, according to the company.
