AnaSpec has developed a solid support AMC resin for facile solid-phase synthesis of peptide-AMC
AnaSpec has introduced AMC resins for SPPS Peptide-7-amino-4-methylcoumarin conjugates (peptide-AMCs) which are widely used as fluorogenic substrates to study proteases.
The preparation of peptide-AMCs usually involves solution phase synthesis.
However, up until now no AMC solid support resin for SPPS has ever been reported.
AnaSpec's solution attaches N-Fmoc-7-aminocoumarin-4-acetic acid (Fmoc-Aca-OH) to Wang resin.
After Fmoc removal, peptides are synthesised on the amino group of the linker using standard SPPS Fmoc chemistry, followed by cleavage with trifluoroacetic acid.
The crude peptide-7-aminocoumarin-4-acetic acid conjugates (peptide-ACAs) undergo facile decarboxylation under mild conditions to afford peptide-AMCs.
Fmoc-ACA-Wang resin, AMC resins preloaded with Asp (D) and Arg for use in SPPS, as well as Fmoc-ACA-OH are now available from AnaSpec.
