This technical note describes how metabolites can be separated from each other using Fortis Technologies’ Diphenyl stationary phase.
Metabolites within HPLC cause probably the most problems along with polar analytes in terms of the ability to separate and resolve them from either the parent ion or each other.
Subtle changes in a functional group or the position of a functional group, loss or gain in an atom or functional group can all be difficult for traditional C18 chemistries to differentiate.
Fortis Technologies has produced an application note on the ability to separate these metabolites from each other and/or the parent compound in a simple way with the company’s Diphenyl stationary phase.
Producing three mechanisms for analytes to interact with including a large steric term helps gain the much needed selectivity so often missing.
Available as a 1.7um particle for use in UHPLC for ultimate sensitivity, or as a analytical and prep particle, Fortis Diphenyl offers a uncomplicated method of analysis for metabolites leaving the ability to run ‘generic screens’ still intact.
To read the full report, please click on the link above.
