TAP Biosystems has developed another method of using microcarriers with its ambr micro bioreactor system.
The technique enables scientists to produce uniform microcarrier samples and perform automated media exchange to rapidly optimise parameters for culturing adherent cell lines on microcarriers, thus shortening timelines for successfully scaling up manufacturing of vaccines and cell therapies.
Scientists at TAP have utilised the ambr workstation to develop an automated method for providing highly consistent dispensing of microcarriers to multiple 10-15mL ambr microbioreactors.
This enabled rapid testing of up to 24 cell-specific culture parameters in parallel including stirring, media formulation or feed strategies to determine the optimum conditions for cell attachment, growth rate and vaccine titre, for example.
The latest method, which has been developed using Vero adherent cells attached to Cytodex 1 microcarriers, allows a 20% media exchange to be performed at any time of the day or night on 24 ambr vessels in approximately four hours.
Since the method allows even microcarrier distribution, scientists can then study up to 24 different parameters in parallel, simultaneously.
This reduces reliance on spinner flasks and bench top bioreactors, saving vaccine and potentially many months of scale-up process development work.
Dr Barney Zoro, ambr product manager at TAP Biosystems said: “There is a great deal of interest in developing vaccines using attachment dependent Vero cells and microcarriers as they allow cell propagation in bioreactors rather than roller bottles. The use of bioreactors can provide greater process control, resulting in high-titre vaccine production.
“However, the main issue is how to accurately mimic bioreactor conditions as producing evenly distributed microcarriers and performing media exchange can be problematic using spinner flasks and benchtop bioreactors. Our new method for microcarrier culture using the ambr system means scientists can begin their experiments with uniform microcarrier samples and perform media exchange without interruption of stirring.”
Zoro added: “Being able to assess different parameters, as well as perform process development in weeks rather than months when using ambr will save scientists valuable time, enabling them to rapidly scale up an optimum cell culture process for more affordable vaccines or as a quick response to unexpected situations such as a pandemic threat.”
The method and results of the study are available in a technical application note, which can be requested via this link.