Olympus has expanded its range of cell total internal reflection fluorescence microscope (TIRF) illuminators and accessories with the one-line illuminator (cellTIRF-1L), which complements the high-end, 4- and 2-line (cellTIRF MITICO 4L/2L) models.
Ideally suited for use with laser combiners, the cellTIRF-1L with motorised TIRF angle control offers flexibility for system builders.
It can also be used with 3rd party lasers and enables multi-fluorescence samples to be sequentially imaged at the same penetration depth.
Control is managed by software integration or via the Olympus Real-Time Controller (RTC) for accurate live cell imaging.
TIRF imaging is used across a wide spectrum of disciplines to investigate events occurring at cell surfaces. CellTIRF illuminators are designed to provide support both precise imaging and easy operation.
Features include highly advanced optics, integrated laser safety systems and high-speed control of penetration depth and laser lines via the RTC.
High end systems include ultra-sensitive truly simultaneous multi-colour TIRF microscopy, with up to four laser channels and independent beam paths.
Models are complemented by a portfolio of specially designed TIRF objectives, including the world’s highest numerical aperture (NA – 1.7) APO 100X OHR, allowing extreme TIRF angles, and the UAPON 150xO TIRF, which combines extreme magnification and high numerical aperture (1.45) with a correction collar and DIC compatibility.
Through outstanding optics matched with high-end motorised TIRF devices, the Olympus system offers a system enabling easy access to super-resolution microscopy, performed using single-molecule localisation techniques.
The range is also fully compatible with the modular IX3 series of inverted microscope frames for extra flexibility flexibility. Such ‘open source’ frames enable bespoke systems with interchangeable optical modules and accessories, thanks to an accessible infinite light path.
One accessory, the Z-drift compensation system (ZDC), enables instant focusing even at high magnifications with dedicated TIRF objectives.
Ensuring constant and stringent focusing over long imaging periods, the ZDC is perfect for time-lapse experiments and complements the workflow-orientated cellVivo incubation system which is equipped with a laser interlock - blocking lasers as soon as the incubator is opened for safe handling.
TIRF illuminator controls and applications are seamlessly integrated into Olympus cellSens software for fast and flexible visualisation of intracellular molecular events with speed, sensitivity and resolution.