Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is a technique for genome-wide profiling of DNA-binding proteins and histone modifications. Allied with next-generation sequencing (NGS) technology, Chromatrap provides a simple and easy to use ChIP format.
Compatible with high and low cell numbers, validated on both transcription factor (TF) and epigenetic mark identification in primary and secondary cell lines.
The availability of Chromatrap ChIP-seq in a 96-well plate allows for high-throughput analysis and, for the first time, provides an unrivalled tool for studying complex, co-ordinated gene regulation and epigenetic mechanisms on a global scale.
This application note demonstrates the utility of the Chromatrap ChIP-seq kit to elucidate the genome-wide binding patterns of nuclear receptor binding events in human adenocarcinoma cells; highlighting the fast multiplex capability alongside massively parallel NGS.
Chromatrap ChIP-seq assays now enable unbiased, high resolution, low noise amplification for genome-wide understanding of enriched protein-DNA regulatory networks.