Size Determination of Vitamin B1 by Dynamic Light Scattering
10 Apr 2017
An application report from Anton Paar details an experiment to show how to determine the most suitable concentration for a DLS measurement of a vitamin B1 sample.
Vitamin B1, or thiamine, is an essential mineral that helps the body access energy by providing elements needed for processing carbohydrates. It also helps maintain the central nervous system's operations. A deficiency in vitamin B1 may result in weight loss, weakness, irregular heart rate and emotional disturbances. The small size and low molecular weight of vitamin B1 makes it an ideal sample for identifying the concentration limits of DLS.
Dynamic light scattering (DLS) is a physics technique used for characterising the size of particles and molecules in suspension and solution. The technique is based on the measurement of the time-dependent fluctuations in the intensity of the scattered light signals. The particles are responsible for the scattering of the light, while it is the random motion of the particles that produces the fluctuations in that scattering. By measuring the scattering, the particles' velocity can be derived, and from that velocity the hydrodynamic diameter of the particles can be calculated by using the Stokes–Einstein equation.
An important factor for making good DLS measurements is using the right concentration. The concentration of particles must be high enough to produce a good scattering intensity. On the other hand, if the concentration is too high, then multiple scattering may occur, which can produce an erroneous result.