The Kapa long-range PCR system from Anachem combines Taq DNA polymerase and an engineered archaeal (Type B) DNA polymerase processing proofreading capability.
The two-enzyme system is designed specifically to support long-range and/or sensitive PCR from complex targets.
It is said to deliver three-to-four times higher fidelity than Taq polymerase.
The Kapa long-range PCR system is said to be ideally suited for PCR amplification of long targets up to 20kb and/or PCR using low amounts of template DNA.
It is also claimed to improve results in standard short- and mid-range PCR amplification, production of PCR products to be used for ligation into 3'-T-overhang cloning vectors and targeted sample enrichment for next-generation DNA sequencing.
This enzyme is also available with Hotstart technology for improved specificity during prolonged bench-top setup or liquid handling.
In the hot-start formulation, the enzyme is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step.
This eliminates spurious amplification products resulting from non-specific priming events during reaction setup and initiation, and increases overall reaction efficiency.
Anachem is offering free samples of Kapa long-range DNA polymerases for a limited period.