Sage-N Research has announced that a research institute dedicated to finding cures for human disease has implemented the Sage-N Sorcerer Enterprise proteomics platform.
The Sanford-Burnham Medical Research Institute (SBMRI) will use the system to enhance the speed of data searches and increase data generation for its proteomics projects, which range from the identification of proteins in gel bands to quantitative total proteome and total phosphoproteome analyses.
SBMRI's Proteomics Facility offers liquid chromatography-tandem mass spectrometry (LC-MS/MS) based proteomics services to support the institute's researchers.
Over recent years the facility has used a variety of search engines to undertake database searches and has recently chosen to implement the Sage-N Sorcerer Enterprise platform for its large-scale analyses.
The facility selected the platform for its ability to offer increased accuracy and throughput while also being able to run the most rigorous algorithms needed for successful pathway proteomic studies.
In addition, the Socerer Enterprise is said to be easy to use and enables researchers to conduct validation of search data in Trans-Proteomic Pipeline (TPP) format.
Since implementing the Sorcerer Enterprise platform, the SBMRI Proteomics Facility has been provided with enhanced search speeds, increased accuracy of analyses and the ability to export data in several formats.
The facility has also improved its process for the identification of peptides, including phosphopeptides, following the development of new algorithms.
In addition, using a decision tree MS/MS method, the platform's Versasearch software provides quality results on these data files, which contain both collision-induced dissociation (CID) and electron transfer dissociation (ETD) spectra.
The proteomics facility's team has been provided with full aftercare support, as well as the opportunity to collaborate with Sage-N's personnel to develop improved tools for the platform.