Cambio has announced that it will be supplying Epicentre’s ARTseq ribosome profiling kits in the UK.
Produced by Epicentre, ARTseq (Active mRNA Translation) ribosome profiling kits enable users to create RNA sequence libraries from ribosome-protected mRNA.
The technique is used to investigate translational control, measure gene expression, identify translation start sites and predict protein abundance.
The procedure involves the isolation of monosomes using a rapid and simplified size-exclusion spin column method, specifically designed for purifying and separating nucleic acids or proteins.
By incorporating enzymatic steps, the gel purification step is reduced. This means that use of the kit is fast, scalable, and does not require special, time-consuming equipment such as gradient stations, ultracentrifuges, or fraction collectors.
The ArtSeq kit is available for both yeast and mammalian samples.
Twelve index primers are included in the ARTseq ribosome profiling kits for the creation of barcoded libraries.
Kits are compatible with Illumina Cluster kits for single read, paired-end and multiplex sequencing using the Illumina GAII, HiSeq and MiSeq sequencers.