Sartorius Stedim Biotech (SSB) has reconfigured its Sartobind membrane chromatography capsules to recover large molecular weight proteins, such as blood factors and conjugated proteins or viruses and virus-like particles more effectively.
A range of capsule sizes with a 4mm bed height is now available from the 1mL nano unit up to the new 2.5L jumbo version.
The capsules’ upstream flow channels have been optimised, and an internal core forms a miniaturised downstream channel, resembling that in existing 8 mm bed height capsules.
The new Q, S and salt-tolerant STIC PA ion exchanger capsules, all with a 4mm bed height, increase dynamic binding capacity by approximately 15% and reduce void volumes by approximately 40% compared with their predecessors, while maintaining high flow rates of 10 to 30 membrane volumes per minute.
By improving breakthrough behaviour, subsequent polishing steps to remove DNA, HCP, aggregates and viruses from recombinant proteins are significantly more reliable and, as less buffer is used, Sartobind also reduces operational costs.
“Traditionally, membrane adsorbers have been using available filter housings, often ignoring chromatographic process parameters, such as back-mixing effects and elution volumes. This new generation of membrane adsorber capsules takes these specific requirements into account and reflects substantial progress for bind and elute applications,” said Fischer-Frühholz, membrane chromatography expert at SSB.
SSB has also added 400mL and 800mL Sartobind Q, S and Phenyl capsules with an 8mm bed height to its portfolio. These optimised capsules increase dynamic binding capacity up to 48% when compared to adsorbers installed in filter housings.