SpyLine is the newest addition to Sigma's RNA product offerings and is an excellent tool for rapidly identifying the knockdown of gene expression for functional profiling of biological processes
Sigma-Aldrich introduces the SpyLine Poly A+ capture kit (product codes: SPY1 and SPY4).
"The SpyLine Poly A+ Capture Kit simplifies knockdown measurements and gene expression analysis by allowing capture and amplification of mRNA in a single plate," said Keith Jolliff, strategic marketing director at Sigma-Aldrich.
"We are very excited about the sensitivity and reproducibility of the kit, making it an excellent addition to our RNA product offering".
The kit features the SpyLine Poly A+ Capture Plate, a 96-well PCR plate coated with oligo(dT) for selective capture of poly A+ mRNA from crude mammalian cell lysates.
Reagents can be added directly to this capture plate for subsequent RT-PCR or real-time quantitative RT-PCR cycling and analysis.
In addition, the 96-well format is suitable for automation.
High throughput protocols are available for the Biomek FX Workstation.
