The new GenomePlex Single Cell WGA Kit supports whole genome amplification from a single cell, resulting in a million-fold amplification yielding microgram quantities of genomic DNA
Providing researchers with a product to amplify genomic DNA from a single cell will help to advance cancer research, genetic profiling and preimplantation genetic diagnostics.
The GenomePlex Single Cell WGA kit will ease the concerns of limited patient source material and provide sufficient DNA for downstream studies.
This kit is the latest addition to the GenomePlex whole genome amplification family of products, and includes all of the reagents necessary for cell lysis and whole genome amplification.
"The launch of the new kit greatly expands the research applications of the GenomePlex whole genome amplification technology," said Keith Jolliff, director of strategic marketing in the research biotech business unit at Sigma-Aldrich.
"Not only will this kit advance our understanding of cell biology, but it also has broad applications in the study of various diseases including cancer".
The GenomePlex single cell whole genome amplification kit can be used in numerous applications, and is suitable for use with a variety of cells, including fibroblast amniotic cells, renal cancer cells, plant, epithelial, leukemia, and polycarbonate fixed cells.
After purification, amplified DNA can be analysed in a manner similar to any genomic or chromosomal DNA sample.
A number of downstream applications may be performed, including QPCR, STR analysis, SNP analysis, comparative genomic hybridisation (CGH), micro arrays, and other genotyping analysis.
The GenomePlex single cell whole genome amplification kit is derived from the proprietary amplification method that is based upon random fragmentation of the genome into a series of overlapping, short templates.
The resulting shorter DNA strands are efficiently primed and amplified to generate a library of DNA fragments with defined 3' and 5' termini, the OmniPlex library.
This library is replicated using a linear, isothermal amplification in the initial stages, followed by a limited round of geometric (PCR) amplifications.
