Premier Biosoft International has released version 5.10 of Beacon Designer with advanced support for multiplexing, crucial for diagnostic assay development
Beacon Designer is Premier Biosoft International's oligo design tool for real time qRT-PCR and is the only tool available that supports most of the popular qPCR chemistries, it says.
"With the release of Beacon Designer Version 5.10, Premier Biosoft International has taken a step further in making a comprehensive, sophisticated and powerful qPCR oligo design tool available to thousands of labs all over the world," says Kay Brown, VP business development and marketing.
"We are particularly pleased with the biologist designed user interface and comprehensive support for multiplexing." Beacon Designer can design SYBR Green primers, TaqMan probes, LNA spiked TaqMan probes, Fret probes or molecular beacons for real time qRT-PCR and Nasba assays.
Beacon Designer also enables the design of compatible probes for proven SYBR Green assays, resulting in cost saving.
The most notable feature of Beacon Designer is the use of innovative and sophisticated algorithms to ensure assay success.
Primers are designed avoiding regions of significant homology, identified by automatically interpreting BLAST search results.
Primer efficiency is enhanced by designing them in template-structure-free regions.
For multiplexing, Beacon Designer screens millions of primer probe combinations to design primer probe sets for single tube multiplex reactions.
Whether the need is to incorporate well-proven sets for some of the templates, or include reference or housekeeping genes for normalisation purposes in the assay, Beacon Designer is fully equipped.
