Bio-Rad Laboratories has announced the use of its MicroRotofor cell for first-dimension protein separation
Bio-Rad Laboratories, a multinational manufacturer and distributor of life science research products and clinical diagnostics, today announced the use of its MicroRotofor cell for first-dimension protein separation prior to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
This new strategy equips researchers in their quest to uncover low-abundance proteins for biomarker discovery.
For first-dimension separation prior to SDS-PAGE, it has been demonstrated in research at the University of Reading (UK) that the MicroRotofor cell offers several advantages over traditional 2-D gel electrophoresis for proteomics.
These include running samples in native or denaturing conditions and separation of proteins that are insoluble in gel-based separation methods.
Also, unlike with some other chromatographic techniques, prefractionated samples can be directly analysed on mini gels without including any concentration steps, thus speeding and facilitating the prefractionation workflow prior to LC-MS/MS analysis.
The MicroRotofor cell is a preparative isoelectric focusing device that fractionates complex protein samples in free solution.
Whether used alone or as a part of a separation or purification scheme, the MicroRotofor cell offers rapid, simple, and effective fractionation and enrichment of proteins from complex protein mixtures.
The majority of proteomics research, whether gel-based or another approach is used, requires fractionation to increase protein resolution for downstream experimentation.
The MicroRotofor cell fractionates by liquid-phase IEF, which is particularly beneficial for proteins that are insoluble or do not separate well in other gel-based IEF media.
