INTEGRA has published a whitepaper that discusses how observing good pipetting practice helps to achieve better results in serial dilution assays.
Preparation of reproducible serial dilution assays, especially in 384-well plates, is a challenging task because they require many pipetting actions and are highly dependent on an operator's technique and experience.
However, observing good pipetting practice helps to improve reproducibility and accuracy.
In this whitepaper the author focuses on a selection of good pipetting techniques, which if maintained offer the best chance of increasing and maintaining the reproducibility of your serial dilutions. Mixing is a key component of dilution protocols.
You can download the white paper from this page.
