The proposed method describes the simultaneous determination of the two amino acids by differential pulse polarography at the dropping mercury electrode (DME)
After the degradation of biological samples (eg, milk, wool, etc), it is often important to know the cystine/cysteine ratio.
The proposed method describes the simultaneous determination of the two amino acids by differential pulse polarography at the dropping mercury electrode (DME).
Milk and sodium caseinate produced from milk have high protein contents.
They have to be analysed in alkaline solution to prevent precipitation of proteins on the separation column.
The determination limits are approx 50ug/l for cysteine and approx 1mg/l for cystine.
The polarographic determination of cystine and cysteine with Metrohm's devices is easy and straightforward: just choose the method in the built-in database of your 797 VA Computrace and start the determination using the MVA-2 which consists of the 797 VA Computrace and two 800 Dosinos.
Or the MVA-3 system for fully automated analysis of small series of samples consisting of the 797 VA Computrace, a 813 compact autosampler, two 800 Dosinos, a 731 relay box and two 772 pump units.
