Leica AM Tirf MC (multi-colour, total internal reflection fluorescence) system allows the entire fluorescence excitation spectrum to now be used for real-time visualisation of live cell dynamics
Leica's unique scanner sensor automatically matches the Tirf angle at all wavelengths to the required penetration depth, and positions the laser so that the Tirf penetration depth remains constant even when the wavelength is changed.
Researchers using Leica's Tirf technology benefit from a unique level of convenience as well as from reproducible scientific results.
The Leica AM Tirf MC integrates four solid-state lasers for the excitation of fluorophores at wavelengths from 405 to 632nm.
The system features extremely short switching times and an ultra-high synchronised frame rate.
For the scientist, this means excellent flexibility for experiments with multiple fluorophores.
The system is particularly useful for exploring molecular interaction at cell membranes and molecular interaction of proteins and receptors involved in transport mechanisms.
With the Leica AM Tirf MC, a scientist can examine single molecules near the cell membrane, study co-localisation and vesicle transport, and combine Tirf and fast Fret analysis.
