BIOO Scientific offers a variety of services using a number of different RNAi technologies including synthetic siRNA and vector-based approaches to help facilitate the progress of drug development
Bioo Scientific is offering an in vivo small RNA testing service to speed up the process of developing small RNAs as drugs compounds.
The cellular RNA interference (RNAi) pathway can be used to specifically regulate gene expression.
RNAi has found utility in the drug identification and development pipeline.
The complete and integrated workflow includes the following:.
Synthetic small RNA formulations using lipid or conjugation technologies.
Manufacturing and administration of shRNA.
Directed or systemic delivery methods available.
Service to analyse gene inhibition, distribution and toxicology in animal tissues (pharmacodynamics and pharmacogenomics).
Customised report containing all the experimental plan, results and conclusions.
Small RNA formulations and chemistry services: unlike cell culture experiments, in vivo delivery is much more challenging.
Using lipid, proteins and novel delivery strategies research has demonstrated that siRNA can be more effectively delivered in vivo to target locations.
It is now possible to direct siRNA to target organs or cells using antibody directed approaches.
Bioo offers siRNA formulations and conjugation services for delivering siRNA and miRNA molecules to the desired cell types and organs.
Lipid formulations can be designed using our formulation screen for the desired organs or may be developed according to your specifications.
Delivery and dosing: small RNA can be directly injected or administered using numerous delivery routes into target locations.
For example, they may be directly injected into subcutaneous xenograph tumors or the brain using specialized techniques.
It has also been found that inhalation is a useful delivery approach for getting small RNAs into the lung.
Delivery routes can be customised to direct the delivery of the small RNAs into the desired location.
Dosing will be dependant upon the goals of the experiments and the formulations that are used, but in general it uses between 5-20ug/g.
Bioo typically uses 1-3 doses for most initial experiments, however this can be modified based on the customers' needs.
Dosing can be administered using pumps, via syringe or using a catheter.
Following the administration of the small RNA molecules, collection of specific body fluids can be performed to specifications.
Also other tests and measurements, can be performed such as: tumour measurements, blood testing and measurements, analysis of cytokine levels in serum, histological embedding of tissue and analysis of protein reduction/siRNA uptake.
Histological embedding of tissue and analysis of protein reduction/siRNA uptake:.
The fluorescence microscopy technique utilises functional, fluorescently labelled siRNA.
Accumulation of the fluorescent signal within the cells in a fixed tissue section (detected by fluorescence microscopy) indicates that siRNA delivery has occurred.
This is a powerful method for detecting the delivery of small RNA; it can be used to optimize and confirm the delivery of small RNAs into cells.
Immunohistochemistry is a complementary technique used for in vivo studies to measure the biological effects of small RNAs at the protein level.
In this technique, fixed tissue sections from small RNA treated animals are probed with labeled antibody directed against the targeted gene.
To confirm silencing, the amount of antibody binding to target small RNA transfected samples are compared to the antibody binding in samples treated with negative control.
