Efficiently delivery siRNA and miRNA into mouse organs using a novel non-toxic blend of fat-soluble naturally-occurring hydrophobic molecules
The advanced formulation enables users to perform siRNA and miRNA delivery in vivo for target validation and drug target identification purposes.
After formulating siRNA with In Vivo RNA RNA-Porter, the RNA becomes stable in serum thus, preventing the need for modifications for stability thus reducing the cost for synthesis.
The procedure only takes about one hour of preparation time and can be performed in any standard laboratory setting.
Simply, mix the siRNA with RNA-Porter, freeze at -80C for ten minutes evaporate the organic solvent using a nitrogen gas stream and the siRNA is ready.
Simply hydrate and injected.
Dried siRNA/RNA-Porter cake is stable for extended periods of time at -20C and thus preparation of the small RNAs can be done days or weeks before the injection is performed.
The delivery route can be focused so that more efficient uptake and reduction may be obtained in your desired destination.
For example, siRNA may be directly injected into a subcutaneous tumor or into a specific organ using surgical procedures.
Alternatively, injection of the siRNA formulated in this agent has been shown to give efficient gene reduction in the liver, lung and kidney.
