Bio-Rad Laboratories has announced availability of technical bulletin 5339A, which compares the desalting of proteins using the Profinia protein purification system against a manual desalting method
The bulletin explains how the mechanism of buffer exchange between the most commonly used methods - dialysis and gel filtration chromatography - is not the same.
Dialysis will usually produce a more concentrated product than gel filtration, whereas desalting by gel filtration using the Profinia protein purification system, an automated low-pressure chromatography system, offers substantial time savings, an automated scalable process in addition to significant advantages of greatly decreased buffer consumption.
Written by Bio-Rad application scientists, the two-page technical bulletin, provides a comprehensive description of the methods used, a discussion of the results, and a conclusion.
The bulletin also includes supporting figures, chromatograms, diagrams, tables, and graphics.
