Agilent has introduced its High Sensitivity Protein 250 kit for the Agilent 2100 bioanalyser, which delivers more sensitivity than silver-stained SDS-polyacrylamide gel electrophoresis (SDS-Page)
The new kit also provides quantitation capability along with the other advantages of a microfluidic lab-on-a-chip based kit.
The High Sensitivity Protein Kit, designed for both pharmaceutical QA/QC and life sciences research experiments, detects proteins down to as little 1pg/ul, comparable to or better than silver-staining slab gels.
The kit features a dynamic range of four orders of magnitude for quantitation and covers a sizing range from 10-250kDa.
For QA/QC applications, users can detect a 0.05-percent impurity while visualising the main target with reliable quantitation.
"This is the latest example of our programme to extend the sensitivity, speed, low sample consumption, ease of use and cost savings of the multi-purpose 2100 bioanalyser system to additional life science applications," said Knut Wintergerst, Agilent's electrophoresis marketing manager.
"We think researchers will immediately recognise the advantages of replacing traditional silver-staining slab gels with a faster, more-reproducible and quantitative method".
The new kit features a direct labelling reaction that is highly reproducible.
Separation, quantitation and purity measurements can be performed in a single step, and typical throughput for QA/QC is ten samples per hour.
Each High Sensitivity Protein 250 kit can be used to analyse 100 samples.
It includes labeling dye and reagents, ten microfluidic chips (each capable of running ten samples), bioanalyser separation reagents, and user documentation.
The assay runs on the standard Agilent 2100 bioanalyser system.
The new kit is available immediately.
