The HT Parp/apoptosis assay is an Elisa which semi-quantitatively detects PAR deposited onto immobilised histone proteins (via Parp activity) in a 96-well format
Trevigen launches high-throughput Parp/apoptosis assays ideal for measuring the activity of Parp in 96-well plates.
Cell extracts can be obtained using our newly-developed, higher-throughput procedure before, during and after apoptosis induction.
During apoptosis, Parp-1 which catalyses the NAD dependent addition of poly (ADP-ribose) (PAR) onto various cytoplasmic and nuclear proteins, is cleaved from its enzymatically active form (about 116kDa), to its inactive form (85kDa).
Trevigen offers two formats of the HT Parp/apoptosis assay: Cat 4684-096-K (colorimetric) and Cat 4685-096-K (chemiluminescent).
Additional histone-coated strip wells (Cat 4677-096-P) are available separately.
