Bio-Rad technical note 5686, 'Optimisation of electroporation conditions for Jurkat cells using the Gene Pulser MXcell electroporation system', offers a comparative study of gene transfer methods
Electroporation is a valuable tool used for nucleic acid delivery since it can be used for a wide variety of cell types including primary and difficult - to-transfect mammalian cells.
While defining electroporation conditions specific to these cell types can be both challenging and time consuming, Bio-Rad's Gene Pulser MXcell electroporation system is making this process easier by enabling scientists to quickly vary multiple electroporation conditions so they can perform experiments with minimal delay.
The technical note describes the optimal electroporation conditions developed for Jurkat cells, a difficult-to-transfect cell line, using the Gene Pulser MXcell electroporation system and Gene Pulser electroporation buffer.
The results were compared to the transfection efficiency of a competitor's instrument in which optimization was performed by varying proprietary buffers using preset protocols.
Finally, a time-course experiment was conducted to assess successful delivery of both siRNA and plasmid DNA into the cells.
The results demonstrate that gene silencing was achieved using the Gene Pulser MXcell system after just four hours; and the silencing persisted for 48 hours.
In addition, results from the four-hour time point suggest that plasmid DNA was successfully delivered directly into the nucleus.
Technical note 5686 is available either from a local Bio-Rad sales office or it may be downloaded from the Bio-Rad website.
