Bio-Rad Laboratories has announced the availability of a poster (number 07-0883) that was displayed at the Association for Biomolecular Resource Facilities Annual Meeting held in February 2008
In the poster, Bio-Rad demonstrates how high efficiency electroporation of several difficult-to-transfect cell lines was achieved using the parameter optimisation capabilities of the Gene Pulser MXcell electroporation system.
Electroporation conditions, including optimal waveform and pulse conditions, were determined in parallel for each cell type.
Results of several difficult-to-transfect cell types, including Huvec (human umbilical vein endothelial cell), Jurkat, and mouse neuroblastoma cells (Neuro-2A) with plasmid DNA or siRNA, are shown.
Data presented in the poster shows that four-hours post electroporation with a GAPDH siRNA, Huvec cells expressed less than 97% of GAPDH mRNA than controls transfected with scrambled siRNA.
In Jurkat cells, significant silencing was also obtained (>88%) as quickly as four hours post transfection and this silencing persisted for 48 hours.
Transfection of Neuro-2A cells with a fluorescently labelled siRNA followed by analysis with flow cytometry resulted in 75% transfection efficiency.
Poster 07-0883 is available either from a local Bio-Rad sales office or it may be downloaded from the company's website.
