Available from ChromSolutions is an application note that details the use of centrigual partition chromatography and the rapid linear scale up of a protein separation
Centrigual partition chromatography (CPC) is a preparative and industrial LC technique.
The main difference in this technique versus HPLC is the absence of a solid support such as silica.
This means that irreversible adsorption to the column of valuable molecules is eliminated.
No denaturations of fragile molecules (eg, proteins peptides or molecules sensitive to hydrolysis) as all the process are carried out in liquid phases.
Injection of 1gram will give a yield of 1gram.
Easy scale up from bench experimentation to industrial scale.
From analysis of petroleum to proteins there are no column changes.
Possibility to inject quite complex mixtures such as natural products and biological matrices.
The technology works by using two non miscible phases one phase is used as a stationary phase and kept in the column by a centrifugal field and another phase is used as the eluent.
Molecules are separated according to their partition coefficient as measured between both phases.
The application note details the use of an aqueous two phase system to purify and scale up of the proteins lysozyme and myoglobin.
