Bio-Rad's Silentmer siRNA duplexes are based on the novel Dicer-substrate siRNA technology, which contributes to their high potency and ability to use very low siRNA concentrations per experiment.
Bio-Rad Laboratories has launched a new siLentMer siRNA search tool, available online.
The Silentmer siRNA search tool offers a way to review Bio-Rad's library of validated siRNA gene targets by searching for a siRNA (by gene symbol, NCBI accession number or Bio-Rad catalogue number) or browsing either the research panels they are categorised into or an alphabetical listing of the target gene symbols.
Potent, well designed siRNAs are crucial to the specific silencing of a target gene.
Additionally, Bio-Rad employs its expertise in gene transfer and PCR by validating each siRNA sequence to ensure its effectiveness in knocking down at least 85% of the target mRNA expression level.
The siLentMer siRNA duplexes offered by Bio-Rad are now categorised into nine panels according to areas of research interest.
The current research panels include angiogenesis; apoptosis; carcinogenesis; cell cycle/checkpoint; chromatin regulation; inflammation; kinases; metabolism; and toxicology/ADME.
Additional panels such as stem cell research, DNA damage/ repair, cardiovascular disease, and others will be added later in the year.
Validated qPCR Primer Pairs.
Bio-Rad also announced the addition of validated qPCR Primer Pairs to their Silentmer product line.
These qPCR primer pairs, used for the validation of the siRNA duplexes, are shipped along with each validated siRNA duplex and are also included in the Silentmer Total Control siRNA transfection kits.
