Bio-Rad Laboratories's technical note 5334 describes a method of large scale removal of albumin and lgG from human serum using the BioLogic DuoFlow chromatography system
The presence of high-abundance albumin and IgG in human serum masks many proteins of interest, especially those analysed by 2-D electrophoresis.
This technical note describes how Affi-Gel Blue and Affi-Gel protein A media provide the high specificity and high degree of efficiency required to meet the needs of large-scale removal of albumin and IgG from human serum.
Combined with the BioLogic DuoFlow chromatography system, separation the method becomes a simultaneous, robust, and automated single-step process.
The technical note (tech note 5334) is available either from a local Bio-Rad sales office or may be downloaded (links provided).
