Bio-Rad Laboratories bulletin 5726A gives an overview of preprogrammed methods for the purification of Protein A and Protein G antibodies using the Bio-Rad Profinia protein purification system
Protein A and G chromatography media are commonly used in antibody purification.
The bulletin describes the preprogrammed Profinia Protein A and G methods using the Profinia protein purification system, for automated affinity purification of monoclonal and polyclonal antibodies.
"Using the Profinia system to purify both monoclonal and polyclonal antibodies not only replaces manual operation that can sometime last hours with gravity flow methods, it also exchanges the acidic buffer of eluted antibody into neutral pH buffer to instantly stabilise the antibody without manual neutralisation," said Wei Liu, product manager for the Bio-Rad Profinia protein purification system.
The system provides a great solution for researchers who need to purify liters of monoclonal antibody from very diluted hybridoma culture and generates pure antibody that could be used for downstream biochemical assays and even amino terminal sequencing of the light or heavy antibody chains.
Availability Bulletin 5726A is available either from a local Bio-Rad sales office or can be downloaded from the Bio-Rad website (linked).
