The far-red fluorescing nuclear counterstain Draq5 has also been validated by many recent publications for a wide range of fixatives, frozen and paraffin-embedded preparations
Due to remarkable spectral properties Draq5 is compatible with the widest range of visible range fluorophores allowing simultaneous and multi-parametric imaging.
One striking example is the combination of a constitutively-expressed GFP-tagged protein in female donor cells, a Y-chromosome Fish Cy3-probe for male recipient cells and Draq5 to demark the nuclei of all cells in mouse cardiac tissue sections (Visconti, 2006).
Usefully, Draq5 works with a full range of fixatives including methanol, acetone, PFA, and PLP and has been shown to stain efficiently both before (Schweitzer, 2005) and more typically after fixation (Grimm, 2004).
Draq5 penetrates deep into tissue sections: staining of 50um-thick fixed sections from rat kidney samples (Toyokawa, 2006) permitted multiple confocal sections and 3D reconstruction for a movie of three parameters.
Draq5 is compatible with wide-field, epifluorescent and confocal microscopes and all available HCS imaging platforms making it a perfect choice for broad application in immunohistochemistry.
