Bio-Rad Laboratories has announced the availability of a new application guide (number 5700 Rev A) for the Bio-Rad Gene Pulser MXcell electroporation system
The 18-page guide is designed to help researchers using the Gene Pulser MXcell to determine the parameters for obtaining efficient transfection of molecules into primary mammalian cells, while maintaining high cell viability.
The guide provides detailed information on all parameters involved in electroporation and their interaction with each other as well as the cell.
It also offers step-by-step instructions on optimizing specific parameters when starting with known electroporation conditions, as well as starting with a cell line in which no conditions exist.
Availability.
Application guide (number 5700 Rev A) is available from a local Bio-Rad sales office and it may be downloaded from the website.
For additional information on optimisation of electroporation parameters and conditions, please see Bio-Rad's technical note 5622, which may also be downloaded online.
Bio-Rad also offers the Gene Transfer Protocol Library, an additional tool designed to help researchers.
The library contains approximately 500 electroprotocols, and has recently been updated.
Electroporation is the introduction of RNA or DNA into cells or protoplasts by the disruption of the cell membrane through exposure to an electric field.
Electroporation is a valuable tool for nucleic acid delivery because it can be used for a wide variety of cell types.
Along with a general movement in the scientific community to use more medically relevant cells, new tools for electroporation have become necessary as more scientists optimize delivery of siRNA, plasmids, or other molecules into mammalian cells, especially primary cells and those that are difficult to transfect.
