Bio-Rad Laboratories has announced the availability of the 'Bio-Plex Pro Human Acute Phase Assay Panel' guide.
The guide (bulletin number 5650) describes the reliable multiplex measurement of nine human acute phase markers.
The guide said Bio-Plex Pro human acute phase assays can deliver accurate and reproducible measurements of the nine commonly studied acute phase markers: a-2-Macroglobulin, CRP, ferritin, fibrinogen, haptoglobin, procalcitonin, SAA, serum amyloid P (SAP), and tissue plasminogen activator - in serum, plasma, and tissue culture supernatant.
A snapshot of panel performance is demonstrated by representative parallelism and linearity of sample dilution in normal human serum and plasma.
The assays are offered for research use as two separate kits to achieve optimal working assay ranges.
The Bio-Plex Pro human acute phase five-plex assay requires a 100-fold sample dilution, and the Bio-Plex Pro human acute phase four-plex assay requires a 10,000-fold sample dilution as required by the physiological ranges of the analytes.
Dividing the targets into panels that require similar dilutions reduces the need to expend assay reagents on multiple dilutions of each sample.