454 Life Sciences and Integrated DNA Technologies have formed an agreement that will see IDT design, synthesise and purify the fusion primers required for certain 454 sequencing applications.
These applications include ultra-deep amplicon sequencing for detection of low-frequency mutations in disease associated genes, and the identification and quantification of common and rare variants within a population.
IDT's online fusion primer design tool uses a gen-specific data base number or a supplied reference sequence to build appropriate 454 Fusionprimers for targeted DNA regions, including individual exons or all exons from one or more genes.
DNA secondary structure, such as hairpin formation, can affect primer performance.
The software takes into account performance metrics of the entire fusion primer rather than simply adding 454 Life Sciences's universal adaptor sequence to an otherwise standard PCR primer.
In additional to 454 Fusionprimer design, IDT has expertise in high-fidelity synthesis and HPLC purification that can aid the researcher in obtaining the best quality data from their 454 sequencing system.
