Bio-Rad Laboratories has announced the availability of a bulletin (number 5737) that describes Itaq fast supermixes with Rox that enable more reliable quantitative PCR (QPCR) results.
The bulletin also provides quantitative graphical proof of the efficacy of the assays.
Itaq fast supermixes with Rox are concentrated and ready-to-use reaction mixtures for real-time QPCR on a variety of instruments including Rox-dependent real-time PCR systems.
These mixes are optimised to deliver maximum PCR efficiency, sensitivity, precision, and robust fluorescent signal using fast or conventional cycling protocols.
The antibody-mediated and hot-start technology employed by Itaq DNA polymerase, sequesters polymerase activity prior to the initial PCR denaturation step.
Upon heat activation, the antibody denatures irreversibly, releasing fully active Itaq DNA polymerase.
This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.
The Rox internal reference dye included in the product is used for normalisation of fluorescent signal and to correct for well-to-well optical variations in Rox-dependent instrumentation.
These supermixes provide a high level of specificity to reduce the occurrence or delay the detection of primer-dimer and other non-specific artifacts.
The bulletin (number 5737) is available from a local Bio-Rad sales office or it can be requested from its website.
