Integrated DNA Technologies (IDT) has launched the first assay products covered by its license under the Probe Manufacturing License programme from Applied Biosystems (ABI).
The Primetime QPCR assays contain dual-labelled probes and primers mixed for use in the 5' Nuclease process.
Primetime QPCR assays use a validated real-time PCR design algorithm.
This real-time PCR design tool allows the researcher to customise the thermodynamic parameters to match specific assay requirements and incorporates genome and transcript information in the primer and probe design.
The amplicons are designed to reduce detection of genomic DNA.
Researchers can evaluate the resulting primer-probe sets, including the sequence of both primers and probe, prior to ordering.
The Primetime QPCR mix contains enough to conduct up to 8,000 assays at standard conditions.
Both primers and probes are analysed by mass spectroscopy to verify their molecular weight, and the dual-labelled probes are purified using HPLC.
The reagents are then mixed and shipped out in three to five days.
