Bio-Rad Laboratories has introduced 12 new protocols for electroporation of human primary cells and primary cell lines.
The cells include T-cells, neutrophils and several B-cell lymphomas.
The conditions for electroporation were developed using Bio-Rad's Gene Pulser MXcell electroporation system and Gene Pulser electroporation buffer.
The protocols can be viewed in Bio-Rad's Gene Transfer Protocol library.
Gene transfer is a general term used to denote transfection or transformation, the transfer of new genetic material into a cell.
Bio-Rad offers several tools for gene transfer including electroporation, lipid-mediated delivery and particle delivery.
Electroporation is a valuable tool for nucleic acid delivery because it can be used for a variety of cell types, including primary cells and cells that are difficult to transfect.
Lipid-mediated delivery is used for mammalian cells.
Particle delivery is useful for plant tissues.
