Bio-Rad Laboratories' CFX384 real-time PCR detection system offers a high-throughput real-time PCR in a 384-well format.
The CFX384 system provides good uniformity and multiplexing capabilities and a dynamic range of detection.
Researchers can run a stand-alone system without a computer or up to four instruments from one computer (either PC or Mac).
The CFX384 uses the Bio-Rad C1000 thermal cycler chassis as the base thermal cycler instrument.
It delivers sensitive, reliable detection for real-time PCR applications such as absolute quantitation, genetic variation analysis and gene expression.
The system reads each well individually with high sensitivity and without cross-talk, to deliver optimal quantitative results.
Multiple data acquisition modes let runs be tailored to suit any application.
The CFX384 real-time PCR detection system provides multiple scan modes for data acquisition so runs can be tailored to different detection chemistries.
The SYBR/FAM only scan mode allows SYBR Green I and single-colour FAM protocols to be completed in less time by collecting data from channel one, for all 384 wells, in just eight seconds.
In multichannel scan mode, the system detects fluorescence from channels one to four, for all 384 wells in less than 20 seconds, enabling four-target discrimination in a single well.
The CFX384 real-time PCR detection system's optics shuttle includes a channel dedicated for single colour FRET (fluorescence resonance energy transfer) experiments, expanding experimental options.
The CFX384's optical system uses long-lasting solid-state technology with filtered LEDs and filtered photodiodes for precise quantitation and target discrimination.
Its PCR detection system can perform four-target multiplex reactions with optimal quantitative results, using low sample volumes.
The CFX384 can be operated in several control configurations including by integrating it into an automation system.
The CFX Manager software lets researchers perform gene expression analysis using multiple reference genes and individual reaction efficiencies.
The CFX384 system can be integrated with the recently introduced CFX Manager Security Edition software, to make it compliant with US FDA 21 CFR Part 11 regulations.
