The 12.765 digital array from Fluidigm Europe is designed to enhance the performance of high-throughput PCR applications including target quantitation, copy number variation and mutation detection.
The Fluidigm 12.765 digital array is an integrated fluidic circuit (IFC) that employs a network of integrated channels and valves to divide a mixture of sample and PCR reagents into 765 replicates.
The chip is specially designed to quantify target sequences very accurately and to detect low-abundance targets that differ by only a base-pair from the wild-type sequence.
With conventional assays, these targets may be otherwise difficult to detect in the sample as a whole.
Using a Fluidigm digital array the sample is diluted, mixed with PCR reagents, and loaded into the chip for partitioning.
This partitioning, in effect, lowers the background within any one partition by a factor equal to the total number of partitions.
After amplification, the target sequences may be quantified by counting the number of positives in relation to the volume of sample loaded.
Incorporating integrated channels, chambers and valves the Fluidigm digital array enhances the efficiency by partitioning a sample into a panel of replicates, without pipetting steps.
The digital array's 12.765 integrated fluidic circuit architecture provides the flexibility to perform digital PCR on any 12 samples and with any licensed PCR chemistry.
Requiring just four steps (transfer, load, run and analyse), the Biomark 12.765 digital array transforms digital PCR from an impractical technique (if performed in microwell plates) into a straightforward routine, said Fluidigm.
