Porvair Filtration has announced that a range of Biovyon products are now available with immobilised Protein A for use in IgG antibody purification.
Biovyon Protein A microplates and columns offer a simple and rapid method for antibody purification.
The products give a consistent recovery of IgG antibody that is independent of the flow rate during loading.
Unlike existing bead-based technologies, there are no slurries to equilibrate and pour into columns.
To use, remove the Biovyon Protein A plate or column from its packaging and add the buffer.
Biovyon Protein A mini and micro columns give consistent recoveries of purified IgG .
The high purity and recoveries are consistent in multiple extractions using the same column or from column to column.
Each mini column has a capacity of approximately 2.5mg IgG (micro - 10ug IgG) and can be reused several times with appropriate washing steps before the performance degrades.
The Biovyon solid state media means that performance is not diminished at flow rates as high as 3ml/min.
The well-to-well consistency of Biovyon Protein A 96-well plates enables the rapid development of automated protocols for IgG screening of cell lines or separations that require IgG capture.
Each well in the product range has a volume of approximately 1ml and can be used in a stand-alone mode in conjunction with a vacuum manifold and collection plate, or as part of a fully automated system.
Biovyon is made from a pharmaceutical grade polyethylene material.
The manufacturing process was developed by Porvair Filtration Group.
Using Activyon technologies, Porvair is pioneering chemical modification and activation of Biovyon to create a range of materials that represent opportunities for ultra-fast purification of biological materials and molecules.
Currently, the Biovyon range includes columns and microplates for solid phase extraction (SPE) and antibody purification.
