Olympus has introduced a 25x water immersion objective specifically designed for multiphoton imaging.
It offers researchers deep-imaging and high resolution with the Olympus Fluoview FV1000MPE multiphoton laser-scanning microscope system.
The Olympus XLPlan N 25x objective has been designed with a numerical aperture (NA) of 1.05 for high-resolution multi-photon microscopy.
It can be used for tissue samples with cover slips and for investigations, such as patch clamping, where the sample cannot be covered.
As a result, higher z-resolution and significantly increased brightness is achieved for all deep imaging applications, compared to similar objectives already on the market, claims Olympus.
With a working distance of 2mm and a 35deg access angle available for patch clamping, this objective is ideal for neurophysiology and related applications.
A wide field of view (27.5) aids researchers in obtaining more information from each multiphoton experiment.
A correction collar compensates for refractive index mismatches, allowing axial and spatial resolution hundreds of microns into living brain samples.
This life science objective for the multiphoton imaging area has been designed to maximise the effectiveness of the multiphoton process, with chromatic aberrations corrected within Ti:Sa IR-laser wavelength range 680 to 1,100nm.
Optical coatings allow the objective to provide high performance in the IR range, with transmittance of more than 82 per cent from 400 to 1,000nm.
This results in at least 5x the normal brightness levels during multiphoton excitation, combined with more efficient collection of emitted light, providing enhanced instrument sensitivity and higher signal-to-noise ratios.
The Olympus XLPlan N 25x (N.A.1.05) objective is designed specifically for deep-specimen imaging, delivering the maximum detection efficiency.
