The University of Middlesex is using the Thermo Scientific Verso cDNA Synthesis Kit and Thermo Scientific Absolute Blue QPCR Master Mix for its cancer biomarker research projects.
The Biomarker (Molecular Pathology) Group at University of Middlesex is using the Thermo Fisher products to detect cancer biomarker expression in different bladder cancer-cell lines such as SCaBER, RT112 and T24.
These kits are being used to detect expression levels of hCG beta-expressing tumour cells, where it has been found that hCG beta is associated with the invading edge of tumours that lead to metastasis.
The researchers are using the Verso cDNA Synthesis Kit to detect the low levels of hCG beta expression from tumour cells.
The Verso cDNA Synthesis Kit can tackle low expression transcripts by utilising a new RT enzyme and optimised priming strategies that combine anchored random hexamers and oligo dT primers in a 3:1 ratio.
After reverse transcribing the mRNA, the laboratory then quantifies the levels of expression with Absolute Blue QPCR Master Mix.
Absolute Blue contains an inert blue dye for easy visualisation of master mix addition.
