Agilent Technologies has introduced the 1290 Infinity liquid chromatography system, designed to deliver more power, speed and sensitivity for enhanced performance in the high-end UHPLC market.
The Agilent 1290 Infinity LC delivers a large analytical power range, enabling users to deploy any particle type, any column dimensions or any mobile and stationary phases.
This range provides a high separation power per time for sub-two-micron and other advanced particle columns.
The system delivers the foundations for method transferability from and to any vendor's UHPLC and HPLC systems.
To complement the performance of the 1290 Infinity LC, Agilent has introduced Zorbax rapid resolution high-definition (RRHD) columns.
The 1.8um particle size delivers optimal resolution and peak definition for both simple and complex separations.
Hardware design and packing processes result in rugged and reliable performance, including stability at the larger power range.
The Zorbax RRHD columns are available in popular Zorbax bonded phases for flexibility and scalability between Agilent instruments.
The Agilent 1290 Infinity LC is designed to drive even higher levels of performance from Agilent LC/MS systems.
The low delay volume, low sample carryover, integrated control and operation with Agilent's Masshunter MS software, plus the ability to perform fast, ultra-high resolution LC separations contribute to high performance.
'This system works well with Agilent Jet Stream inlet technology on our high end 6460 Triple Quadrupole, 6530 Accurate Mass Quadrupole time of flight (TOF), and 6230 TOF MS systems,' said Ken Miller, Agilent's global marketing manager for LC/MS.
'We have demonstrated improved sensitivity by reducing ion suppression and matrix effects.
'We have been able to identify more compounds in complex mixtures as well as reduce method times and boost throughput for screening assays,' he added.
The 1290 Infinity binary pump module reduces background noise, contributing to the system's very high signal-to-noise ratio.
Active damping combines advanced pump engineering with embedded firmware to reduce 'pump ripples' and associated UV noise.
Background noise is further reduced by Agilent's Jet Weaver microfluidic mixing technology, which combines a high gradient mixing efficiency with low delay volume.
The 1290 Infinity LC also features a UV diode array detector (DAD) with high sensitivity.
The module contains a Max-Light cartridge cell with optofluidic waveguides, providing low limit of detection and high signal-to-noise ratio.
In addition, baseline drift is minimised for more reliable and precise peak integration, because compromising refractive index and thermal effects are nearly eliminated.
A programmable slit lets the user optimise sensitivity, linearity and spectral resolution.
The 1290 Infinity autosampler and thermostatted column compartment modules contain a number of usability and high-throughput features, including the ability to configure the system to run more than 2,000 samples per eight-hour shift.
Alternating column regeneration (ACR) reduces cycle time by half compared to single column configuration, and throughput can be maximised further using automatic delay volume reduction, overlapped injections, offline data analysis and external needle wash capabilities.
The autosampler is based on the next generation of flow-through design, providing high precision for both very small and large injection volumes without loop changes.
For an injection, only the injected volume from the sample is needed, since no valuable sample is wasted for flushing purposes.
Inert materials in metering device seal and needle seat combine with reduced hydraulic volume to contribute to very low carryover.
Additionally, in combination with the 1290 Infinity flexible cube module, the needle seat can be automatically backflushed for lowest carry over.
This flexible cube also provides an option for ultra-fast cycle times in the fixed-loop injection mode, offering infinite choices for sample injection.
The system can also be configured for walk-up multi-method operation and automated method development with a choice of eight columns and 26 solvents.
