Premier Biosoft International has released Primerplex version 2.00.
Primerplex version 2.00 designs custom oligos for high-throughput detection of nucleic-acid sequences for Luminex 100, Luminex 200 and Bio-Plex 200 suspension array systems.
The systems are based on Luminex's bead-array based Xmap technology.
Primerplex can now use pre-designed oligos to build a multiplex set.
The user begins by specifying the oligos for each sequence; for the sequences for which pre-designed oligos are not available, Primerplex designs them, checks them for multiplexing and highlights any compatibility issues.
The user can then decide to accept the design or create a separate pool.
This gives the user complete control.
Version 2.00 supports a database of Microplex Xtags (formerly known as Flexmap Tags) for automatic addition of appropriate tags to each sequence.
The tags minimise dimerization and do not fold back on the oligos they are attached to.
In addition, a user can override the program's recommendation and select their own.
The functionality is available for Allele Specific Primer Extension (ASPE) primers and capture probes.
Primerplex is suitable for multiplex nucleic acid applications of Xmap technology such as SNP analysis, pathogen detection, strain typing, haplotyping and gene expression.
The program now also supports loading of rsID sequences, mutations such as DIPs (Deletion/Insertion Polymorphisms) and MNPs (Multiple Nucleotide Polymorphisms) and can simultaneously process multiple SNPs for large genomic sequences, reporting overlapping designs if any occur.
